cell signaling technology 2956 clone d5 1 Search Results


gfp d5 1  (Cell Signaling Technology Inc)
96
Cell Signaling Technology Inc gfp d5 1
Gfp D5 1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cell+signaling+technology+2956+clone+d5+1/GFP+Rabbit+mAb/pm39753546-248-30-32
Average 96 stars, based on 1 article reviews
gfp d5 1 - by Bioz Stars, 2026-09
96/100 stars
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gfp d5 1 2956 rabbit  (Cell Signaling Technology Inc)
94
Cell Signaling Technology Inc gfp d5 1 2956 rabbit
Gfp D5 1 2956 Rabbit, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cell+signaling+technology+2956+clone+d5+1/DyLight+650+Phalloidin/pmc12799961-259-31-49
Average 94 stars, based on 1 article reviews
gfp d5 1 2956 rabbit - by Bioz Stars, 2026-09
94/100 stars
  Buy from Supplier
gfp  (Cell Signaling Technology Inc)
97
Cell Signaling Technology Inc gfp
A. Ratio of 5’-PPP and 5’-P-bearing RNY1 and RNY4 in Jurkat cells 48h post infection <t>with</t> <t>HIV-GFP</t> or in non-infected (NI) Jurkat. Relative 5’-PPP/5’-P RNA levels were determined through differential enzyme digestion followed by qPCR analysis relative to β-actin mRNA. RN7SL1 and U17b are 5’-PPP and 5’-P RNA controls, respectively. B. DUSP11 protein levels measured at different times points after Jurkat T cells infection with HIV-GFP. C. DUSP11 protein levels measured in NI or HIV-GFP-infected CD4 primary cells from 2 different donors 48h post-infection. CD4 T cells were beads-sorted from total PBMC and activated with PHA for 72h prior to infection with HIV-GFP. 48h post-infection, productively infected cells were FACS-sorted according to GFP expression. D. DUSP11 protein levels measured at 6h and 48h after Jurkat T cells infection with WT NL4.3 HIV-1 or the same clone deleted for VPR protein. E. Volcano-plot of differential expressed genes in 3 WT or DUSP11 -/- Jurkat clones. IFN or IFN-I regulated genes (IRG) (annotation according to interferome database) are labelled in purple (dark and light, respectively). A. Bars show mean +/− s.e.m. of n=3 independent experiments. Student’s t-test *p<0.05. B-D . Western Blot representative of n=3 independent experiments. B-D Numbers at the bottom indicate semi-quantification of relative DUSP11/GAPDH levels normalized to NI conditions.
Gfp, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cell+signaling+technology+2956+clone+d5+1/GFP+Antibody/bio_rxiv__773820-170-43-46
Average 97 stars, based on 1 article reviews
gfp - by Bioz Stars, 2026-09
97/100 stars
  Buy from Supplier
d5 1  (Cell Signaling Technology Inc)
96
Cell Signaling Technology Inc d5 1
A. Ratio of 5’-PPP and 5’-P-bearing RNY1 and RNY4 in Jurkat cells 48h post infection <t>with</t> <t>HIV-GFP</t> or in non-infected (NI) Jurkat. Relative 5’-PPP/5’-P RNA levels were determined through differential enzyme digestion followed by qPCR analysis relative to β-actin mRNA. RN7SL1 and U17b are 5’-PPP and 5’-P RNA controls, respectively. B. DUSP11 protein levels measured at different times points after Jurkat T cells infection with HIV-GFP. C. DUSP11 protein levels measured in NI or HIV-GFP-infected CD4 primary cells from 2 different donors 48h post-infection. CD4 T cells were beads-sorted from total PBMC and activated with PHA for 72h prior to infection with HIV-GFP. 48h post-infection, productively infected cells were FACS-sorted according to GFP expression. D. DUSP11 protein levels measured at 6h and 48h after Jurkat T cells infection with WT NL4.3 HIV-1 or the same clone deleted for VPR protein. E. Volcano-plot of differential expressed genes in 3 WT or DUSP11 -/- Jurkat clones. IFN or IFN-I regulated genes (IRG) (annotation according to interferome database) are labelled in purple (dark and light, respectively). A. Bars show mean +/− s.e.m. of n=3 independent experiments. Student’s t-test *p<0.05. B-D . Western Blot representative of n=3 independent experiments. B-D Numbers at the bottom indicate semi-quantification of relative DUSP11/GAPDH levels normalized to NI conditions.
D5 1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cell+signaling+technology+2956+clone+d5+1/BRM+XP+Rabbit+mAb/pm39515329-349-73-81
Average 96 stars, based on 1 article reviews
d5 1 - by Bioz Stars, 2026-09
96/100 stars
  Buy from Supplier
gfp  (Proteintech)
96
Proteintech gfp
A. Ratio of 5’-PPP and 5’-P-bearing RNY1 and RNY4 in Jurkat cells 48h post infection <t>with</t> <t>HIV-GFP</t> or in non-infected (NI) Jurkat. Relative 5’-PPP/5’-P RNA levels were determined through differential enzyme digestion followed by qPCR analysis relative to β-actin mRNA. RN7SL1 and U17b are 5’-PPP and 5’-P RNA controls, respectively. B. DUSP11 protein levels measured at different times points after Jurkat T cells infection with HIV-GFP. C. DUSP11 protein levels measured in NI or HIV-GFP-infected CD4 primary cells from 2 different donors 48h post-infection. CD4 T cells were beads-sorted from total PBMC and activated with PHA for 72h prior to infection with HIV-GFP. 48h post-infection, productively infected cells were FACS-sorted according to GFP expression. D. DUSP11 protein levels measured at 6h and 48h after Jurkat T cells infection with WT NL4.3 HIV-1 or the same clone deleted for VPR protein. E. Volcano-plot of differential expressed genes in 3 WT or DUSP11 -/- Jurkat clones. IFN or IFN-I regulated genes (IRG) (annotation according to interferome database) are labelled in purple (dark and light, respectively). A. Bars show mean +/− s.e.m. of n=3 independent experiments. Student’s t-test *p<0.05. B-D . Western Blot representative of n=3 independent experiments. B-D Numbers at the bottom indicate semi-quantification of relative DUSP11/GAPDH levels normalized to NI conditions.
Gfp, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cell+signaling+technology+2956+clone+d5+1/GFP+tag+Antibody/bio_rxiv__773820-170-43-30
Average 96 stars, based on 1 article reviews
gfp - by Bioz Stars, 2026-09
96/100 stars
  Buy from Supplier
gfp d5 1 rabbit mab  (Cell Signaling Technology Inc)
94
Cell Signaling Technology Inc gfp d5 1 rabbit mab
A. Ratio of 5’-PPP and 5’-P-bearing RNY1 and RNY4 in Jurkat cells 48h post infection <t>with</t> <t>HIV-GFP</t> or in non-infected (NI) Jurkat. Relative 5’-PPP/5’-P RNA levels were determined through differential enzyme digestion followed by qPCR analysis relative to β-actin mRNA. RN7SL1 and U17b are 5’-PPP and 5’-P RNA controls, respectively. B. DUSP11 protein levels measured at different times points after Jurkat T cells infection with HIV-GFP. C. DUSP11 protein levels measured in NI or HIV-GFP-infected CD4 primary cells from 2 different donors 48h post-infection. CD4 T cells were beads-sorted from total PBMC and activated with PHA for 72h prior to infection with HIV-GFP. 48h post-infection, productively infected cells were FACS-sorted according to GFP expression. D. DUSP11 protein levels measured at 6h and 48h after Jurkat T cells infection with WT NL4.3 HIV-1 or the same clone deleted for VPR protein. E. Volcano-plot of differential expressed genes in 3 WT or DUSP11 -/- Jurkat clones. IFN or IFN-I regulated genes (IRG) (annotation according to interferome database) are labelled in purple (dark and light, respectively). A. Bars show mean +/− s.e.m. of n=3 independent experiments. Student’s t-test *p<0.05. B-D . Western Blot representative of n=3 independent experiments. B-D Numbers at the bottom indicate semi-quantification of relative DUSP11/GAPDH levels normalized to NI conditions.
Gfp D5 1 Rabbit Mab, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cell+signaling+technology+2956+clone+d5+1/GFP+Rabbit+mAb/pmc10443793-110-4-19
Average 94 stars, based on 1 article reviews
gfp d5 1 rabbit mab - by Bioz Stars, 2026-09
94/100 stars
  Buy from Supplier

Image Search Results


A. Ratio of 5’-PPP and 5’-P-bearing RNY1 and RNY4 in Jurkat cells 48h post infection with HIV-GFP or in non-infected (NI) Jurkat. Relative 5’-PPP/5’-P RNA levels were determined through differential enzyme digestion followed by qPCR analysis relative to β-actin mRNA. RN7SL1 and U17b are 5’-PPP and 5’-P RNA controls, respectively. B. DUSP11 protein levels measured at different times points after Jurkat T cells infection with HIV-GFP. C. DUSP11 protein levels measured in NI or HIV-GFP-infected CD4 primary cells from 2 different donors 48h post-infection. CD4 T cells were beads-sorted from total PBMC and activated with PHA for 72h prior to infection with HIV-GFP. 48h post-infection, productively infected cells were FACS-sorted according to GFP expression. D. DUSP11 protein levels measured at 6h and 48h after Jurkat T cells infection with WT NL4.3 HIV-1 or the same clone deleted for VPR protein. E. Volcano-plot of differential expressed genes in 3 WT or DUSP11 -/- Jurkat clones. IFN or IFN-I regulated genes (IRG) (annotation according to interferome database) are labelled in purple (dark and light, respectively). A. Bars show mean +/− s.e.m. of n=3 independent experiments. Student’s t-test *p<0.05. B-D . Western Blot representative of n=3 independent experiments. B-D Numbers at the bottom indicate semi-quantification of relative DUSP11/GAPDH levels normalized to NI conditions.

Journal: bioRxiv

Article Title: Y-RNAs Lead an Endogenous Program of RIG-I Agonism Mobilized upon RNA Virus Infection and Targeted by HIV

doi: 10.1101/773820

Figure Lengend Snippet: A. Ratio of 5’-PPP and 5’-P-bearing RNY1 and RNY4 in Jurkat cells 48h post infection with HIV-GFP or in non-infected (NI) Jurkat. Relative 5’-PPP/5’-P RNA levels were determined through differential enzyme digestion followed by qPCR analysis relative to β-actin mRNA. RN7SL1 and U17b are 5’-PPP and 5’-P RNA controls, respectively. B. DUSP11 protein levels measured at different times points after Jurkat T cells infection with HIV-GFP. C. DUSP11 protein levels measured in NI or HIV-GFP-infected CD4 primary cells from 2 different donors 48h post-infection. CD4 T cells were beads-sorted from total PBMC and activated with PHA for 72h prior to infection with HIV-GFP. 48h post-infection, productively infected cells were FACS-sorted according to GFP expression. D. DUSP11 protein levels measured at 6h and 48h after Jurkat T cells infection with WT NL4.3 HIV-1 or the same clone deleted for VPR protein. E. Volcano-plot of differential expressed genes in 3 WT or DUSP11 -/- Jurkat clones. IFN or IFN-I regulated genes (IRG) (annotation according to interferome database) are labelled in purple (dark and light, respectively). A. Bars show mean +/− s.e.m. of n=3 independent experiments. Student’s t-test *p<0.05. B-D . Western Blot representative of n=3 independent experiments. B-D Numbers at the bottom indicate semi-quantification of relative DUSP11/GAPDH levels normalized to NI conditions.

Article Snippet: The following antibodies were used: HIV-1 p24 (mouse monoclonal, Abcam, clone 39/5.4A, #ab9071); MAVS (rabbit polyclonal, Cell Signaling Technology - CST, #3993); DUSP11 (rabbit polyclonal, Proteintech, #10204-2-AP); α-tubulin (mouse monoclonal, Proteintech, clone 1E4C11, #66031-1-Ig); HIV-1 gp120 (sheep polyclonal, gift from Benjamin Chen laboratory); GFP (rabbit monoclonal, CST, clone D5.1, #2956); GAPDH (rabbit monoclonal, CST, clone 14C10, #2118); VPR (rabbit polyclonal, Proteintech, # 51143-1-AP), StrEP-Tag (mouse monoclonal, Qiagen, #34850).

Techniques: Infection, Expressing, Clone Assay, Western Blot

A. Hierarchical clustering and heatmap based on genes from DUSP11 -/- gene signature, differentially expressed between primary cells from 3 different donors, productively (GFP+), or non-productively (GFP-) infected with HIV-GFP. B. Hierarchical clustering and heatmap based on genes from DUSP11 -/- gene signature, differentially expressed between CD4 T cells from non-infected patients and acutely infected non-treated HIV+ patients. C. Western blot (left) and relative quantification (right) showing DUSP11 level in CD4 T cells from HIV+ patients prior and after anti-retroviral treatment. Paired t-test *p<0.05.

Journal: bioRxiv

Article Title: Y-RNAs Lead an Endogenous Program of RIG-I Agonism Mobilized upon RNA Virus Infection and Targeted by HIV

doi: 10.1101/773820

Figure Lengend Snippet: A. Hierarchical clustering and heatmap based on genes from DUSP11 -/- gene signature, differentially expressed between primary cells from 3 different donors, productively (GFP+), or non-productively (GFP-) infected with HIV-GFP. B. Hierarchical clustering and heatmap based on genes from DUSP11 -/- gene signature, differentially expressed between CD4 T cells from non-infected patients and acutely infected non-treated HIV+ patients. C. Western blot (left) and relative quantification (right) showing DUSP11 level in CD4 T cells from HIV+ patients prior and after anti-retroviral treatment. Paired t-test *p<0.05.

Article Snippet: The following antibodies were used: HIV-1 p24 (mouse monoclonal, Abcam, clone 39/5.4A, #ab9071); MAVS (rabbit polyclonal, Cell Signaling Technology - CST, #3993); DUSP11 (rabbit polyclonal, Proteintech, #10204-2-AP); α-tubulin (mouse monoclonal, Proteintech, clone 1E4C11, #66031-1-Ig); HIV-1 gp120 (sheep polyclonal, gift from Benjamin Chen laboratory); GFP (rabbit monoclonal, CST, clone D5.1, #2956); GAPDH (rabbit monoclonal, CST, clone 14C10, #2118); VPR (rabbit polyclonal, Proteintech, # 51143-1-AP), StrEP-Tag (mouse monoclonal, Qiagen, #34850).

Techniques: Infection, Western Blot

A. Ratio of 5’-PPP and 5’-P-bearing RNY1 and RNY4 in Jurkat cells 48h post infection with HIV-GFP or in non-infected (NI) Jurkat. Relative 5’-PPP/5’-P RNA levels were determined through differential enzyme digestion followed by qPCR analysis relative to β-actin mRNA. RN7SL1 and U17b are 5’-PPP and 5’-P RNA controls, respectively. B. DUSP11 protein levels measured at different times points after Jurkat T cells infection with HIV-GFP. C. DUSP11 protein levels measured in NI or HIV-GFP-infected CD4 primary cells from 2 different donors 48h post-infection. CD4 T cells were beads-sorted from total PBMC and activated with PHA for 72h prior to infection with HIV-GFP. 48h post-infection, productively infected cells were FACS-sorted according to GFP expression. D. DUSP11 protein levels measured at 6h and 48h after Jurkat T cells infection with WT NL4.3 HIV-1 or the same clone deleted for VPR protein. E. Volcano-plot of differential expressed genes in 3 WT or DUSP11 -/- Jurkat clones. IFN or IFN-I regulated genes (IRG) (annotation according to interferome database) are labelled in purple (dark and light, respectively). A. Bars show mean +/− s.e.m. of n=3 independent experiments. Student’s t-test *p<0.05. B-D . Western Blot representative of n=3 independent experiments. B-D Numbers at the bottom indicate semi-quantification of relative DUSP11/GAPDH levels normalized to NI conditions.

Journal: bioRxiv

Article Title: Y-RNAs Lead an Endogenous Program of RIG-I Agonism Mobilized upon RNA Virus Infection and Targeted by HIV

doi: 10.1101/773820

Figure Lengend Snippet: A. Ratio of 5’-PPP and 5’-P-bearing RNY1 and RNY4 in Jurkat cells 48h post infection with HIV-GFP or in non-infected (NI) Jurkat. Relative 5’-PPP/5’-P RNA levels were determined through differential enzyme digestion followed by qPCR analysis relative to β-actin mRNA. RN7SL1 and U17b are 5’-PPP and 5’-P RNA controls, respectively. B. DUSP11 protein levels measured at different times points after Jurkat T cells infection with HIV-GFP. C. DUSP11 protein levels measured in NI or HIV-GFP-infected CD4 primary cells from 2 different donors 48h post-infection. CD4 T cells were beads-sorted from total PBMC and activated with PHA for 72h prior to infection with HIV-GFP. 48h post-infection, productively infected cells were FACS-sorted according to GFP expression. D. DUSP11 protein levels measured at 6h and 48h after Jurkat T cells infection with WT NL4.3 HIV-1 or the same clone deleted for VPR protein. E. Volcano-plot of differential expressed genes in 3 WT or DUSP11 -/- Jurkat clones. IFN or IFN-I regulated genes (IRG) (annotation according to interferome database) are labelled in purple (dark and light, respectively). A. Bars show mean +/− s.e.m. of n=3 independent experiments. Student’s t-test *p<0.05. B-D . Western Blot representative of n=3 independent experiments. B-D Numbers at the bottom indicate semi-quantification of relative DUSP11/GAPDH levels normalized to NI conditions.

Article Snippet: The following antibodies were used: HIV-1 p24 (mouse monoclonal, Abcam, clone 39/5.4A, #ab9071); MAVS (rabbit polyclonal, Cell Signaling Technology - CST, #3993); DUSP11 (rabbit polyclonal, Proteintech, #10204-2-AP); α-tubulin (mouse monoclonal, Proteintech, clone 1E4C11, #66031-1-Ig); HIV-1 gp120 (sheep polyclonal, gift from Benjamin Chen laboratory); GFP (rabbit monoclonal, CST, clone D5.1, #2956); GAPDH (rabbit monoclonal, CST, clone 14C10, #2118); VPR (rabbit polyclonal, Proteintech, # 51143-1-AP), StrEP-Tag (mouse monoclonal, Qiagen, #34850).

Techniques: Infection, Expressing, Clone Assay, Western Blot

A. Hierarchical clustering and heatmap based on genes from DUSP11 -/- gene signature, differentially expressed between primary cells from 3 different donors, productively (GFP+), or non-productively (GFP-) infected with HIV-GFP. B. Hierarchical clustering and heatmap based on genes from DUSP11 -/- gene signature, differentially expressed between CD4 T cells from non-infected patients and acutely infected non-treated HIV+ patients. C. Western blot (left) and relative quantification (right) showing DUSP11 level in CD4 T cells from HIV+ patients prior and after anti-retroviral treatment. Paired t-test *p<0.05.

Journal: bioRxiv

Article Title: Y-RNAs Lead an Endogenous Program of RIG-I Agonism Mobilized upon RNA Virus Infection and Targeted by HIV

doi: 10.1101/773820

Figure Lengend Snippet: A. Hierarchical clustering and heatmap based on genes from DUSP11 -/- gene signature, differentially expressed between primary cells from 3 different donors, productively (GFP+), or non-productively (GFP-) infected with HIV-GFP. B. Hierarchical clustering and heatmap based on genes from DUSP11 -/- gene signature, differentially expressed between CD4 T cells from non-infected patients and acutely infected non-treated HIV+ patients. C. Western blot (left) and relative quantification (right) showing DUSP11 level in CD4 T cells from HIV+ patients prior and after anti-retroviral treatment. Paired t-test *p<0.05.

Article Snippet: The following antibodies were used: HIV-1 p24 (mouse monoclonal, Abcam, clone 39/5.4A, #ab9071); MAVS (rabbit polyclonal, Cell Signaling Technology - CST, #3993); DUSP11 (rabbit polyclonal, Proteintech, #10204-2-AP); α-tubulin (mouse monoclonal, Proteintech, clone 1E4C11, #66031-1-Ig); HIV-1 gp120 (sheep polyclonal, gift from Benjamin Chen laboratory); GFP (rabbit monoclonal, CST, clone D5.1, #2956); GAPDH (rabbit monoclonal, CST, clone 14C10, #2118); VPR (rabbit polyclonal, Proteintech, # 51143-1-AP), StrEP-Tag (mouse monoclonal, Qiagen, #34850).

Techniques: Infection, Western Blot